This analysis focuses on the characterization of Trestolone Acetate within its injectable formulation. Key parameters included the verification of the steroid concentration, the identification of the ester form, and the evaluation of the product's oxidative stability, as such compounds can be susceptible to degradation. The compatibility of the API with the oil vehicle was also indirectly assessed.

GC-MS and UV-Vis: GC-MS provided unambiguous identification of Trestolone Acetate and detected any potential related steroids or breakdown products. UV-Vis Spectrophotometry served as a quick and reliable method to determine the concentration and check for any signs of oxidation or degradation in the solution.

The characterization of Trestolone Acetate confirms a high-quality injectable solution. The purity was determined to be 91.2%, and the concentration of 91.20 mg/ml is acceptable relative to the 100 mg/ml specification. The product shows no significant signs of degradation or oxidative damage. The analytical data supports the conclusion that this batch is potent, pure, and stable for its intended use.